Bifidobacterium longum
Research reviewed: Up until 03/2026
Bifidobacterium longum is a dietary supplement with 22 published peer-reviewed studies involving 1,545 participants, researched for IBS & Gut Health, Stress, Anxiety & Mood, Immune Function and 2 more areas.
Evidence at a Glance
Strength is scored by study design, sample size, study type, and outcomes
IBS & Gut Health
ModerateStress, Anxiety & Mood
ModerateImmune Function
StrongClinical trials
ModerateSystematic reviews
WeakResearch Visualised
Visual breakdown of the clinical data.
Study Quality Breakdown
What types of studies were conducted
Participants Per Study
Larger samples = more reliable results
Research Timeline
When the studies were published
All Studies
Detailed breakdown of each trial. Click to expand.
IBS & Gut Health
To evaluate B. longum 35624 (Bifantis) for IBS symptom relief.
Study Type
Randomised, double-blind, placebo-controlled
Purpose
To evaluate B. longum 35624 (Bifantis) for IBS symptom relief.
Dose
10^8 CFU/day
Participants
77 IBS patients
Duration
8 weeks
Results
B. longum 35624 significantly reduced composite IBS symptoms, abdominal pain, and bloating compared to placebo. Anti-inflammatory effects observed via IL-10/IL-12 ratio.
How They Measured It
IBS symptom composite score, abdominal pain, bloating, bowel habits
To determine optimal dose of B. longum 35624 for IBS.
Study Type
Dose-finding, double-blind, placebo-controlled
Purpose
To determine optimal dose of B. longum 35624 for IBS.
Dose
10^6, 10^8, or 10^10 CFU/day
Participants
362 IBS patients
Duration
4 weeks
Results
The 10^8 dose was optimal, significantly improving all IBS symptoms. Higher and lower doses were less effective than 10^8.
How They Measured It
IBS-SSS, adequate relief
Stress, Anxiety & Mood
To evaluate B. longum 1714 on stress and cognitive performance.
Study Type
Randomised, double-blind, placebo-controlled
Purpose
To evaluate B. longum 1714 on stress and cognitive performance.
Dose
10^9 CFU/day
Participants
22 healthy male volunteers
Duration
4 weeks
Results
B. longum 1714 reduced perceived stress scores and cortisol response. Improved visuospatial memory performance in cognitive testing.
How They Measured It
Perceived Stress Scale, cortisol awakening response, cognitive tests (SCWT, cyberball)
To assess B. longum NCC3001 for anxiety and depression in IBS patients.
Study Type
Randomised, double-blind, placebo-controlled
Purpose
To assess B. longum NCC3001 for anxiety and depression in IBS patients.
Dose
10^10 CFU/day
Participants
44 IBS patients with anxiety/depression
Duration
6 weeks
Results
B. longum NCC3001 significantly reduced depression scores and improved quality of life. fMRI showed reduced reactivity to negative emotional stimuli.
How They Measured It
Hospital Anxiety and Depression Scale, IBS-QoL
Immune Function
To evaluate B. longum on immune function and respiratory infections in elderly.
Study Type
Randomised, double-blind, placebo-controlled
Purpose
To evaluate B. longum on immune function and respiratory infections in elderly.
Dose
2 × 10^9 CFU/day
Participants
66 elderly residents ≥65 years
Duration
20 weeks
Results
B. longum significantly increased fecal IgA levels and NK cell activity. Trend towards reduced respiratory infection incidence.
How They Measured It
Incidence of respiratory infections, immune markers (IgA, NK cell activity)
To assess B. longum supplementation on inflammatory markers in elderly.
Study Type
Randomised, double-blind, placebo-controlled
Purpose
To assess B. longum supplementation on inflammatory markers in elderly.
Dose
10^9 CFU/day
Participants
60 elderly volunteers
Duration
8 weeks
Results
B. longum reduced serum IL-6 and TNF-alpha levels. Increased relative abundance of Bifidobacterium in fecal samples.
How They Measured It
hs-CRP, IL-6, TNF-alpha, fecal microbiome composition
To evaluate B. longum on allergic rhinitis symptoms.
Study Type
Randomised controlled trial
Purpose
To evaluate B. longum on allergic rhinitis symptoms.
Dose
5 × 10^9 CFU/day
Participants
40 patients with allergic rhinitis during pollen season
Duration
8 weeks
Results
B. longum significantly improved Total Nasal Symptom Score and reduced serum IgE levels compared to placebo.
How They Measured It
Total Nasal Symptom Score, IgE levels
Clinical trials
To investigate the effects of Bifidobacterium longum in strain specific effects of probiotic supplementation on serum amino acid profiles in alzheimer's disease: a randomized, double blind, placebo controll
Study Type
Randomized, double-blind, placebo-controlled
Purpose
To investigate the effects of Bifidobacterium longum in strain specific effects of probiotic supplementation on serum amino acid profiles in alzheimer's disease: a randomized, double blind, placebo controll
Dose
Bifidobacterium longum (dose not specified)
Participants
60 participants
Duration
Duration not specified
Results
across all primary outcome measures. Probiotic supplementation, particularly with B. longum, significantly improved serum amino acid profiles in patients with mild to moderate Alzheimer's disease. These findings support the potential of specific probiotic strains to address metabolic imbalances in AD through gut-brain axis modulation. Trial Registration: IRCT number: 20210513051277N1 (2021-05-27).
How They Measured It
See study for outcome measures
To investigate the effects of Bifidobacterium longum in effects of kefir on symptoms, sleep, and gut microbiota in children with adhd: a randomised controlled trial.
Study Type
Randomized, double-blind, placebo-controlled
Purpose
To investigate the effects of Bifidobacterium longum in effects of kefir on symptoms, sleep, and gut microbiota in children with adhd: a randomised controlled trial.
Dose
Bifidobacterium longum (dose not specified)
Participants
Participants not specified
Duration
Duration not specified
Results
Evidence indicates the gut microbiome may be altered in ADHD, suggesting that targeting gut bacteria could alleviate symptoms. This study examined the effects of kefir supplementation on ADHD symptoms, sleep, attention, and gut microbiome composition in children diagnosed with ADHD.
How They Measured It
See study for outcome measures
To investigate the effects of Bifidobacterium longum in the impact of bifidobacterium longum ccfm1112 on chronic constipation: a randomised, double-blind, placebo-controlled study.
Study Type
Randomized, double-blind, placebo-controlled
Purpose
To investigate the effects of Bifidobacterium longum in the impact of bifidobacterium longum ccfm1112 on chronic constipation: a randomised, double-blind, placebo-controlled study.
Dose
1112 g
Participants
Participants not specified
Duration
Duration not specified
Results
iliensis, Blautia sp. SC05B48, Anaerobutyricum hallii, and Streptococcus salivarius, was also significantly elevated. Furthermore, it elevated fecal levels of linoleic acid, gamma-aminobutyric acid (GABA), and arachidonic acid, while increasing L-glutamic acid and decreasing adenosine in serum. Our research findings provide evidence that the intake of B. longum CCFM1112 can alleviate constipation.
How They Measured It
See study for outcome measures
To investigate the effects of Bifidobacterium longum in impact of prebiotics and postbiotics in the management of constipation: analysis of their differentiated modulation of intestinal microbiota.
Study Type
Randomized, double-blind, placebo-controlled
Purpose
To investigate the effects of Bifidobacterium longum in impact of prebiotics and postbiotics in the management of constipation: analysis of their differentiated modulation of intestinal microbiota.
Dose
Bifidobacterium longum (dose not specified)
Participants
24 subjects with constipation were supplemen
Duration
Duration not specified
Results
ugh NextDext™ influenced more microbial groups and restored microbial groups associated with opioid use, the species modulated by ABB C24 reverted microbial alterations consistently reported in constipation, suggesting an added value of the postbiotic ABB C22®. Both could benefit different profiles of constipation sufferers. This study was registered in ClinicalTrials.gov: NCT06738433.
How They Measured It
See study for outcome measures
To investigate the effects of Bifidobacterium longum in exploring neurotransmitter regulation following probiotic supplementation in adults with subthreshold depression: a secondary analysis of a randomized
Study Type
Randomized, double-blind, placebo-controlled
Purpose
To investigate the effects of Bifidobacterium longum in exploring neurotransmitter regulation following probiotic supplementation in adults with subthreshold depression: a secondary analysis of a randomized
Dose
Bifidobacterium longum (dose not specified)
Participants
Participants not specified
Duration
Duration not specified
Results
This secondary analysis examined changes in plasma concentrations of gamma-aminobutyric acid (GABA) and serotonin following multi-species probiotic supplementation versus placebo in Australian adults with subthreshold depression and investigated the potential relationships between neurotransmitter changes and mental health outcomes.
How They Measured It
See study for outcome measures
To investigate the effects of Bifidobacterium longum in perioperative oral immunonutrient regulation of intestinal barrier and gut microbiota in patients with gastric cancer, a randomized controlled clinica
Study Type
Clinical study
Purpose
To investigate the effects of Bifidobacterium longum in perioperative oral immunonutrient regulation of intestinal barrier and gut microbiota in patients with gastric cancer, a randomized controlled clinica
Dose
282 mg
Participants
58 participants
Duration
Duration not specified
Results
: Immunonutrients may attenuate surgery-induced intestinal barrier damage in gastric cancer patients by modulating gut microbiota diversity, enriching beneficial taxa, and suppressing pathogenic bacteria.
How They Measured It
See study for outcome measures
To investigate the effects of Bifidobacterium longum in effects of supplementation with two probiotic strains (lactobacillus helveticus and bifidobacterium longum) on hormonal status, oxidative stress, and
Study Type
Randomized, double-blind, placebo-controlled
Purpose
To investigate the effects of Bifidobacterium longum in effects of supplementation with two probiotic strains (lactobacillus helveticus and bifidobacterium longum) on hormonal status, oxidative stress, and
Dose
Bifidobacterium longum (dose not specified)
Participants
90 women
Duration
8 weeks
Results
, these findings suggest that 8 weeks of probiotic supplementation may alleviate oxidative stress, modulate certain hormonal factors, and reduce inflammation in women with PCOS. Trial registration, the current study was registered in the Iranian Registry of Clinical Trials on 30 March 2024 (ID: IRCT20121216011763N62) ( https://irct.behdasht.gov.ir/trial/76067 ). https://irct.behdasht.gov.ir/trial/
How They Measured It
See study for outcome measures
To investigate the effects of Bifidobacterium longum in feasibility, safety, and impact of the probiotics lactiplantibacillus plantarum and bifidobacterium longum subspecies infantis in papua new guinean in
Study Type
Clinical trial
Purpose
To investigate the effects of Bifidobacterium longum in feasibility, safety, and impact of the probiotics lactiplantibacillus plantarum and bifidobacterium longum subspecies infantis in papua new guinean in
Dose
Bifidobacterium longum (dose not specified)
Participants
Participants not specified
Duration
Duration not specified
Results
Childhood mortality in low- or middle-income countries (LMICs) remains a major public health concern, with infections being a leading cause of infant death. Probiotics have shown promise in reducing infection-related morbidity and mortality in preterm infants, but their use in newborns born at or near term in LMICs requires further investigation.
How They Measured It
See study for outcome measures
Systematic reviews
To investigate the effects of Bifidobacterium longum in probiotics in term infants: clinical impact of infant-type bifidobacteria: a systematic review and meta-analyses.
Study Type
Systematic review and meta-analysis
Purpose
To investigate the effects of Bifidobacterium longum in probiotics in term infants: clinical impact of infant-type bifidobacteria: a systematic review and meta-analyses.
Dose
Bifidobacterium longum (dose not specified)
Participants
Participants not specified
Duration
Duration not specified
Results
iratory tract infections. However, the evidence is limited by study heterogeneity and a lack of long-term follow-up data. Further high-quality randomized controlled trials with larger sample sizes and standardized outcome measures are needed to clarify both short- and long-term effects of ITB probiotic administration in neonates and infants. This trial was registered at PROSPERO as CRD42024507608.
How They Measured It
See study for outcome measures
To investigate the effects of Bifidobacterium longum in meta-analytical insight on probiotic metabolites and inflammatory markers in diabetes.
Study Type
Systematic review and meta-analysis
Purpose
To investigate the effects of Bifidobacterium longum in meta-analytical insight on probiotic metabolites and inflammatory markers in diabetes.
Dose
Bifidobacterium longum (dose not specified)
Participants
Participants not specified
Duration
Duration not specified
Results
play a crucial role in modulating immune responses. Probiotics and synbiotics are increasingly explored for their potential to mitigate inflammation via microbiota-targeted mechanisms. This study aims to evaluate the effects of probiotic and synbiotic supplementation on inflammatory markers and microbial metabolites in individuals with type 1 and type 2 diabetes through meta-analytical techniques.
How They Measured It
See study for outcome measures
To investigate the effects of Bifidobacterium longum in the role of probiotics in modulating rankl in osteoporosis: a systematic review of animal interventional studies and rcts.
Study Type
Systematic review
Purpose
To investigate the effects of Bifidobacterium longum in the role of probiotics in modulating rankl in osteoporosis: a systematic review of animal interventional studies and rcts.
Dose
Bifidobacterium longum (dose not specified)
Participants
Participants not specified
Duration
Duration not specified
Results
Osteoporosis is a major global health concern characterized by reduced bone mass and structural deterioration. The RANK/RANKL/OPG signaling pathway plays a pivotal role in bone remodeling, particularly in osteoclastogenesis. Emerging evidence suggests that probiotics may modulate this pathway through the gut-bone axis, offering potential therapeutic benefits for bone loss.
How They Measured It
See study for outcome measures
To investigate the effects of Bifidobacterium longum in the impact of gut microbiota modulation on responses to immune checkpoint inhibitors in cancer.
Study Type
Systematic review and meta-analysis
Purpose
To investigate the effects of Bifidobacterium longum in the impact of gut microbiota modulation on responses to immune checkpoint inhibitors in cancer.
Dose
Bifidobacterium longum (dose not specified)
Participants
642 participants
Duration
Duration not specified
Results
sponse rates (OR 2.91, 95% CI 1.48-5.73). Microbiota diversity indices were consistently higher in responders than in non-responders. Collectively, gut microbiota composition and its modulation significantly impact the therapeutic efficacy and toxicity profile of ICIs. These findings highlight the translational potential of microbiome-based biomarkers and interventions in optimizing immunotherapy.
How They Measured It
See study for outcome measures
To investigate the effects of Bifidobacterium longum in comparative effectiveness and safety of probiotics with psychotropic potential in mental health benefits in irritable bowel syndrome: a systematic rev
Study Type
Systematic review and meta-analysis
Purpose
To investigate the effects of Bifidobacterium longum in comparative effectiveness and safety of probiotics with psychotropic potential in mental health benefits in irritable bowel syndrome: a systematic rev
Dose
Bifidobacterium longum (dose not specified)
Participants
Participants not specified
Duration
Duration not specified
Results
The use of probiotics as a treatment for irritable bowel syndrome (IBS) is gaining attention, with recent studies indicating that certain probiotics or combinations may have mental health benefits for patients with IBS.
How They Measured It
See study for outcome measures
To investigate the effects of Bifidobacterium longum in the effectiveness of probiotics as an adjunct therapy in patients under mechanical ventilation: an umbrella systematic review and meta-analysis.
Study Type
Systematic review and meta-analysis
Purpose
To investigate the effects of Bifidobacterium longum in the effectiveness of probiotics as an adjunct therapy in patients under mechanical ventilation: an umbrella systematic review and meta-analysis.
Dose
Bifidobacterium longum (dose not specified)
Participants
Participants not specified
Duration
Duration not specified
Results
, B. salivarius , Pediococcus pentosaceus , Lactococcus raffinolactis , B. infantis , B. bifidum , Streptococcus thermophilus , Ligilactobacillus salivarius , L. lactis , B. lactis , Saccharomyces boulardii , L. rhamnosus GG, L. johnsonii , L. casei , S. faecalis , Clostridium butyricum , Bacillus mesentericus , L. sporogenes , S. boulardii , L. paracasei , B. subtilis , and Enterococcus faecium .
How They Measured It
See study for outcome measures
To investigate the effects of Bifidobacterium longum in efficacy of probiotics regimens for helicobacter pylori eradication: a systematic review, pairwise, and network meta-analysis of randomized controlled
Study Type
Systematic review and meta-analysis
Purpose
To investigate the effects of Bifidobacterium longum in efficacy of probiotics regimens for helicobacter pylori eradication: a systematic review, pairwise, and network meta-analysis of randomized controlled
Dose
Bifidobacterium longum (dose not specified)
Participants
Participants not specified
Duration
Duration not specified
Results
the risks of chronic gastritis, peptic ulcer diseases, and the incidence of gastric cancer. However, antibiotic resistance and adverse effects led to the emergence of alternative treatments such as probiotics supplementation. This systematic review and network meta-analysis aims to assess the efficacy and safety of incorporating probiotics into the various eradication regimens for H. pylori.
How They Measured It
See study for outcome measures
Frequently Asked Questions
Common questions about Bifidobacterium longum research
There are currently 22 peer-reviewed studies on Bifidobacterium longum (Bifidobacterium longum), involving 1,545 total participants. Research covers IBS & Gut Health, Stress, Anxiety & Mood, Immune Function and 2 more areas. The overall evidence strength is rated as Strong.
The evidence is currently rated as "Strong Evidence". This rating is based on study design quality (randomisation, blinding, placebo controls), sample sizes, study types (15 human studies), and reported outcomes.
Bifidobacterium longum has been researched for: IBS & Gut Health, Stress, Anxiety & Mood, Immune Function, Clinical trials, Systematic reviews. Each area has its own body of evidence which you can explore in the study breakdowns above.
Yes, 15 out of 22 studies are human trials. Human trials carry more weight in our evidence scoring system.
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