Proline and Lysine
Research reviewed: Up until 03/2026
Proline and Lysine (L-Proline and L-Lysine (Essential Collagen Synthesis Amino Acids)) is a dietary supplement with 5 published peer-reviewed studies involving 480 participants, researched for Collagen Synthesis Support, Wound Healing, Cardiovascular Support.
Evidence at a Glance
Strength is scored by study design, sample size, study type, and outcomes
Collagen Synthesis Support
ModerateWound Healing
ModerateCardiovascular Support
WeakResearch Visualised
Visual breakdown of the clinical data.
Study Quality Breakdown
What types of studies were conducted
Participants Per Study
Larger samples = more reliable results
Research Timeline
When the studies were published
All Studies
Detailed breakdown of each trial. Click to expand.
Collagen Synthesis Support
To perform control analysis of collagen synthesis by glycine, proline, and lysine in bovine chondrocytes and assess implications for supplementation.
Study Type
In vitro mechanistic study
Purpose
To perform control analysis of collagen synthesis by glycine, proline, and lysine in bovine chondrocytes and assess implications for supplementation.
Dose
Various amino acid concentrations in cell culture
Participants
In vitro (bovine chondrocytes)
Duration
Laboratory study
Results
The main cause of procollagen inefficiency is protein misfolding primarily due to glycine scarcity, with moderate deficiency of proline and lysine also limiting collagen synthesis. High glycine and moderate proline/lysine concentrations promote maximum collagen output.
How They Measured It
Procollagen synthesis rates, amino acid concentration-response curves, protein misfolding analysis
To assess the effect of vitamin C-enriched gelatin (containing proline and lysine) on collagen synthesis markers during intermittent exercise.
Study Type
Randomised clinical trial
Purpose
To assess the effect of vitamin C-enriched gelatin (containing proline and lysine) on collagen synthesis markers during intermittent exercise.
Dose
5, 15, or 48 g gelatin enriched with 48 mg vitamin C
Participants
8 healthy males
Duration
3 days
Results
Gelatin supplementation dose-dependently increased serum P1NP (collagen synthesis biomarker). Plasma proline, hydroxyproline, and hydroxylysine peaked 1 hour post-supplementation. The 15 g dose doubled P1NP. Proline and lysine are critical rate-limiting precursors.
How They Measured It
Serum P1NP (procollagen I N-terminal propeptide), plasma proline, hydroxyproline
To review proline precursors and collagen synthesis, examining biochemical challenges of nutrient supplementation and wound healing.
Study Type
Review
Purpose
To review proline precursors and collagen synthesis, examining biochemical challenges of nutrient supplementation and wound healing.
Dose
Various proline precursor forms
Participants
Review
Duration
Various
Results
Proline is required for hydroxylation to hydroxyproline in the collagen triple helix. Adequate proline supply is critical for collagen crosslinking and structural integrity. Lysine hydroxylation to hydroxylysine is essential for collagen crosslinks and thermal stability.
How They Measured It
Review of proline metabolism, collagen hydroxylation, and wound healing requirements
Wound Healing
To evaluate a spray containing L-proline, L-leucine, L-lysine, and glycine combined with sodium hyaluronate to manage chemo/radiotherapy-induced oral mucositis.
Study Type
Open-label clinical study
Purpose
To evaluate a spray containing L-proline, L-leucine, L-lysine, and glycine combined with sodium hyaluronate to manage chemo/radiotherapy-induced oral mucositis.
Dose
Topical spray: L-proline, L-leucine, L-lysine, glycine + sodium hyaluronate
Participants
Patients with oral mucositis from chemotherapy/radiotherapy
Duration
4 weeks
Results
The collagen precursor spray significantly reduced oral mucositis grade and pain scores. Oral function (eating, speaking) improved significantly. The combination of L-proline, L-lysine, glycine, and hyaluronate supported mucosal healing.
How They Measured It
WHO oral mucositis grade, pain VAS, oral function assessment
Cardiovascular Support
To review the role of lysine in lipoprotein(a) binding and cardiovascular disease prevention.
Study Type
Mechanistic review
Purpose
To review the role of lysine in lipoprotein(a) binding and cardiovascular disease prevention.
Dose
High-dose lysine supplementation (1-6 g/day)
Participants
Review
Duration
Various
Results
Lysine residues in arterial walls are binding sites for lipoprotein(a) [Lp(a)], a cardiovascular risk factor. High-dose lysine may competitively inhibit Lp(a) binding to arterial walls. Proline and lysine together with vitamin C support arterial collagen synthesis and repair.
How They Measured It
Review of lipoprotein(a) binding to lysine residues in arterial walls, Lp(a) assays
Frequently Asked Questions
Common questions about Proline and Lysine research
There are currently 8 peer-reviewed studies on Proline and Lysine (L-Proline and L-Lysine (Essential Collagen Synthesis Amino Acids)), involving 480 total participants. Research covers Collagen synthesis support, Wound healing, Connective tissue health and 1 more areas. The overall evidence strength is rated as Moderate.
The evidence is currently rated as "Moderate Evidence". This rating is based on study design quality (randomisation, blinding, placebo controls), sample sizes, study types (2 human studies), and reported outcomes.
Proline and Lysine has been researched for: Collagen synthesis support, Wound healing, Connective tissue health, Cardiovascular support. Each area has its own body of evidence which you can explore in the study breakdowns above.
Yes, 2 out of 8 studies are human trials. Human trials carry more weight in our evidence scoring system.
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