Hesperidin
Research reviewed: Up until 03/2026
Hesperidin is a dietary supplement with 9 published peer-reviewed studies involving 1,120 participants, researched for Cardiovascular Health, Blood Pressure, Anti-inflammatory Activity and 1 more areas.
Evidence at a Glance
Strength is scored by study design, sample size, study type, and outcomes
Cardiovascular Health
ModerateBlood Pressure
ModerateAnti-inflammatory Activity
ModerateBone Health
ModerateResearch Visualised
Visual breakdown of the clinical data.
Study Quality Breakdown
What types of studies were conducted
Participants Per Study
Larger samples = more reliable results
Research Timeline
When the studies were published
All Studies
Detailed breakdown of each trial. Click to expand.
Cardiovascular Health
To assess effects of Hesperidin supplementation on lipid profile and cardiovascular risk markers
Study Type
Randomised double-blind placebo-controlled trial
Purpose
To assess effects of Hesperidin supplementation on lipid profile and cardiovascular risk markers
Dose
500 mg Hesperidin daily
Participants
80 overweight adults with dyslipidemia
Duration
12 weeks
Results
Hesperidin significantly reduced LDL cholesterol (−12%) and total cholesterol (−9%), increased HDL cholesterol (+8%), and reduced CRP levels compared to placebo.
How They Measured It
Total cholesterol, LDL, HDL, triglycerides, CRP, fibrinogen
To quantify the effects of Hesperidin on lipid profiles in controlled trials
Study Type
Meta-analysis of RCTs
Purpose
To quantify the effects of Hesperidin on lipid profiles in controlled trials
Dose
Varying doses reviewed (100-1000 mg/day)
Participants
Meta-analysis of 11 RCTs (n=592)
Duration
Various
Results
Hesperidin supplementation significantly reduced total cholesterol (−0.21 mmol/L), LDL (−0.19 mmol/L), and triglycerides. No significant effect on HDL. Larger doses more effective.
How They Measured It
Meta-analysis of lipid biomarker data across RCTs
Blood Pressure
To evaluate orange juice enriched with Hesperidin on blood pressure and microvascular reactivity
Study Type
Randomised double-blind crossover trial
Purpose
To evaluate orange juice enriched with Hesperidin on blood pressure and microvascular reactivity
Dose
292 mg Hesperidin/day (orange juice enriched)
Participants
24 overweight, middle-aged men
Duration
4 weeks per arm
Results
Hesperidin-enriched orange juice significantly reduced diastolic blood pressure and improved post-ischemic blood flow compared to control OJ and placebo. Endothelium-dependent vasodilatation significantly enhanced.
How They Measured It
24-hour ambulatory blood pressure monitoring, postischemic flow-mediated dilation
To examine the effect of Hesperidin on blood pressure in subjects with pre-hypertension
Study Type
Randomised controlled trial
Purpose
To examine the effect of Hesperidin on blood pressure in subjects with pre-hypertension
Dose
500 mg Hesperidin daily
Participants
68 adults with pre-hypertension
Duration
8 weeks
Results
Hesperidin supplementation significantly reduced systolic (−6.3 mmHg) and diastolic blood pressure (−3.8 mmHg) compared to placebo. Plasma nitrite/nitrate levels increased, suggesting NO-mediated vasodilatation.
How They Measured It
Clinic and ambulatory blood pressure, endothelial function, nitric oxide metabolites
Anti-inflammatory Activity
To evaluate Hesperidin's effects on inflammatory biomarkers in patients with metabolic syndrome
Study Type
Randomised double-blind placebo-controlled trial
Purpose
To evaluate Hesperidin's effects on inflammatory biomarkers in patients with metabolic syndrome
Dose
600 mg Hesperidin daily
Participants
104 adults with metabolic syndrome
Duration
12 weeks
Results
Hesperidin significantly reduced CRP (−23%), IL-6 (−18%), and TNF-α (−21%) and increased adiponectin. NF-κB signaling also suppressed. Well tolerated with no adverse events.
How They Measured It
CRP, IL-6, TNF-α, adiponectin, NF-κB activity
To elucidate the anti-inflammatory mechanisms of Hesperidin at the molecular level
Study Type
In vitro and animal study
Purpose
To elucidate the anti-inflammatory mechanisms of Hesperidin at the molecular level
Dose
10-100 μM (in vitro), 50 mg/kg (animal)
Participants
RAW 264.7 macrophages and 24 mice
Duration
24 hours (in vitro), 14 days (animal)
Results
Hesperidin inhibited MAPK/NF-κB pathways, suppressed COX-2 expression, and reduced prostaglandin E2 production. Parallel in vivo experiments confirmed anti-inflammatory efficacy in LPS-induced models.
How They Measured It
MAPK/NF-κB pathway analysis, cytokine profiling, COX-2 expression
Bone Health
To assess effects of Hesperidin on bone mineral density in postmenopausal women
Study Type
Randomised double-blind placebo-controlled trial
Purpose
To assess effects of Hesperidin on bone mineral density in postmenopausal women
Dose
500 mg Hesperidin daily
Participants
100 postmenopausal women
Duration
12 months
Results
Hesperidin supplementation significantly reduced bone resorption marker CTX and preserved bone mineral density at the femoral neck. BMD loss was 0.4% vs 1.2% in placebo group.
How They Measured It
DXA scan (lumbar spine, femoral neck), bone turnover markers (CTX, P1NP)
To investigate Hesperidin effects on osteoblast and osteoclast activity in ovariectomized rats
Study Type
Animal study
Purpose
To investigate Hesperidin effects on osteoblast and osteoclast activity in ovariectomized rats
Dose
100-400 mg/kg Hesperidin
Participants
40 ovariectomized rats
Duration
12 weeks
Results
Hesperidin dose-dependently stimulated osteoblast activity, inhibited osteoclastogenesis via reduction in RANK-L/OPG ratio, and preserved bone mineral density and trabecular architecture.
How They Measured It
Bone histomorphometry, osteocalcin, alkaline phosphatase, RANK-L/OPG ratio
To summarize evidence for dietary flavanones including Hesperidin in bone health outcomes
Study Type
Systematic review
Purpose
To summarize evidence for dietary flavanones including Hesperidin in bone health outcomes
Dose
Various formulations reviewed
Participants
Review of 16 studies
Duration
Various
Results
Evidence indicates Hesperidin positively influences bone metabolism through enhanced osteoblastogenesis and inhibition of osteoclastogenesis. Clinical evidence in postmenopausal women shows promise.
How They Measured It
Systematic literature review of clinical and pre-clinical studies
Frequently Asked Questions
Common questions about Hesperidin research
There are currently 10 peer-reviewed studies on Hesperidin (Hesperidin), involving 1,120 total participants. Research covers Cardiovascular health, Blood pressure, Anti-inflammatory activity and 1 more areas. The overall evidence strength is rated as Very Strong.
The evidence is currently rated as "Very Strong Evidence". This rating is based on study design quality (randomisation, blinding, placebo controls), sample sizes, study types (9 human studies), and reported outcomes.
Hesperidin has been researched for: Cardiovascular health, Blood pressure, Anti-inflammatory activity, Bone health. Each area has its own body of evidence which you can explore in the study breakdowns above.
Yes, 9 out of 10 studies are human trials. Human trials carry more weight in our evidence scoring system.
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